Innovative Lab Products for the Life Sciences The Bullet Blender™ Family 全世界性价比最好的均质器，美国全进口产品全新诞生！  国内产品的价格,人民币RMB¥3万多！超级实惠的产品, 同样享受美国全进口产品的品质！     广泛用于动物组织、生物样品、食品,化妆品等均质处理， 特别适合于植物, 微生物检测样本的制备。

The easiest and coolest way  to lyse cells and homogenize tissue No cross contamination worries! And samples stay cool! The Bullet Blender™ Homogenize 24 samples at a time! The BULLET BLENDER™ enables you to homogenize, disrupt, or lyse 24 tissue or cell culture samples at a time.  Load the samples into polypropylene microcentrifuge tubes, then place them in the BULLET BLENDER™.  The "bullets" in the "blender" vigorously strike all the sample tubes  simultaneously for a few minutes.  Now available with Air Cooling™ to keep samples even cooler! > Fast, Consistent Results > No Cross Contamination > Samples Stay Cool > Convenient > Risk Free Applications:   Homogenizing cell cultures such as yeast and E. coli to tough tissues such as cartilage, tumors, heart muscle and hair, as well as other organs such as liver, spleen, kidney, brain, lung, and skin from mice or rats.  Other organisms like Drosophila, C. elegans, and plants are amenable to Bullet Blender™ processing.    It's also used for extended vortexing or grinding, like chemical extractions or in applications such as phenol/chloroform extraction and blood processsing. DNA RNA Protein from plant samples from carcinoma samples from E. coli samples Genomic DNA samples in agarose gel stained with ethidium  bromide. Lanes 1-4 = Tobacco leaf DNA λ = λ phage Hind III digest Lanes 5-8 = Rice leaf DNA samples Lanes 1-7= RT-PCR products UV/Vis data indicates RNA quality   SDS-PAGE gel stained with Coomassie Blue wc = whole cell sf = soluble fraction Left two lanes control Right two lanes induced “It's a very useful device for making tissue lysates.  We no longer use sonication for DNA, RNA and protein preparation from multiple tissue samples.” Prof. Michael Sherman, Boston University School of Medicine   “The Bullet Blender has made our clinical lab much more efficient; we get consistent results in less time.  We cannot imagine going back to hand vortexing for our whole blood extractions.” Kimberly Dumont, supervisor, Dartmouth Reference Lab   “I can count on it for fast consistent results.” Dr. Denis Schewe, Mt. Sinai Medical Center,  New York City   “The Bullet Blender™ lets us reduce variability and user error and increase productivity.” Dr. Scott Gridley, Director of Protein Sciences, Blue Sky Biotech, Inc., Worcester, MA. The Bullet Blender™ Family The BULLET BLENDER™ enables you to disrupt or homogenize many samples at a time, by incorporating patented striking technology.  The sample tubes are forcefully agitated thousands of times per minute, inducing vigorous motion of the substances inside the tubes. Simply place your sample, some buffer, and optionally some beads in standard polypropylene tubes.  Place the tubes in the BULLET BLENDER™ and run for a few minutes.  Afterwards, place the sample tubes in a centrifuge to separate the lysate.  Because the samples are in separate tubes and no probes contact the samples, cross contamination is very easy to prevent.  The patented design requires very little power, so the motor will last for years.  Also, the motor will not heat up significantly, so the samples stay cool.   The addition of beads in the sample tubes allows disruption or thorough  homogenization of cells and tissues.  Tune the extent of disruption by adjusting the speed. Air Cooling™ is an option on the microtube model and standard on the 50 mL tube model.  The Air Cooling™ entails fans which exchange air with their surroundings; they have nothing that actually chills the instrument.  Air Cooling™ is useful if you're running several sample cycles in a row or if your samples are extra sensitive to heating up.  Normally, the samples in the Bullet Blender™ only heat up a few degrees and stay quite cool.  However, if you're running a second batch of samples soon afterwards, they will heat up a little more because the Bullet Blender™ is starting out slightly warmer. The Air Cooling™ keeps the Bullet Blender™ cooler so that the first batch of samples heat up a little less and subsequent batches are kept just about as cool as the first batch. Click here to see sample protocols or answers to frequently asked questions (FAQs). The Bullet Blender™ BLUE 50 Similar to the original Bullet Blender™, this model holds up to 9 of 50 mL tubes at a time.  The sample, lysis buffer, and beads should occupy no more than 15 ml of any tube to allow plenty of room for the sample and beads to slosh around.  While this version is not as good for tough tissue, it is ideal for cell culture and larger volume soft tissue samples, such as entire rat lungs.  This model requires BD Falcon™ or Krackeler brand polypropylene 50 ml tubes; tubes that are a slightly different size may not work properly.  Because of the longer processing times (about 3X longer than with microcentrifuge tubes), this version is only available with Air Cooling™. The 3 BULLET BLENDER™ models   Bullet Blender™ Bullet Blender™ BLUE Bullet Blender™ BLUE 50 Capacity 24 Tubes 24 Tubes 9 Tubes Tubes 1.5 to 2.0mL  polypropylene microtubes (e.g. Axygen brand) 50mL polypropylene (e.g. Falcon™) Maximum Fill Volume 1.0 to 1.5mL 15mL with Air Cooling™? No Yes Yes Applications vortexing, homogenizing cell cultures and soft to very tough tissue, extractions vortexing, homogenizing cell cultures and soft tissue, extractions     > Fast, Consistent Results The BULLET BLENDER™ prepares up to 24 samples in just minutes.  All of the samples are automatically homogenized the same, so there is very little variation from sample to sample.  See the Protocols pages for preparation times and speeds. > No Cross Contamination or Sample Loss There are no probes or blades to cause cross contamination or sample loss.   The sample tubes are kept closed during agitation, as the cells or tissue are disrupted.  There are no probes to clean between samples. > Samples Stay Cool The instrument uses very little power due to the unique, patented design, so there is very little heat build up in the instrument or inside the sample tubes.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years. > Convenient to Use Simply place your cells or tissue, and optionally some beads, in standard tubes, and load the tubes into the BULLET BLENDER™.   Set the duration (typically several minutes) and speed (vigorousness).  There are no probes to clean.  It is much quieter than a sonicator and the samples do not heat up more than a few degrees! > Risk Free Purchase The BULLET BLENDERS™ come with a 30 day money back guarantee and a 2 year warranty. The simple, reliable design enables the BULLET BLENDERS™ to sell for one half to one fifth the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker cooler technique. “This past January [2008] we purchased a Bullet Blender from Next Advance.  ...  Let me begin by saying that we are extremely satisfied with the product! We have been able to implement it into a number of protocols that we use in our lab.  Other labs in our department have also taken a liking to the unit and have been using it along side our lab.” Dr. Kyle Bender, Queen's University, Kingston, Ontario, CANADA   “Using the Dounce homogenizer, I can process eight tumors in an hour and a half.  With the Bullet Blender™, I can homogenize twenty in fifteen minutes.” Dr. Alejandro Adam, Albany Medical Center, New York FEATURES Compact - The footprint is only 8in. (20cm) wide by 10in. (25cm) deep High Throughput The Bullet Blender™ and Bullet Blender™ BLUE : twenty-four 1.5mL to 2.0mL microcentrifuge tubes Bullet Blender™ BLUE 50: nine 50mL polypropylene tubes. Easy  - User friendly with only two settings to select: time and speed (up to 5 minutes for microcentrifuge tubes, up to 15 min. for 50 mL tubes.   Momentary on by pressing the "Start" button. Versatile - Use the Bullet Blender™ in different laboratory environments, including cold rooms (4°C to 60°C, avoid condensing conditions).  Requires only an electrical outlet for operation. CE certified 24V power supply, shipped with the proper plug configuration for your wall outlets. Air Cooling™ on  "BLUE" models prevents the instrument from heating up when processing multiple rounds of samples.  The COOLEST homogenizer available! Patented striking technology. Built in the USA for years of trouble free use. Warranty: two years parts and labor, three years on the motor. 30-day money back guarantee.   BULLET BLENDER™ Brochure BULLET BLENDER™ FAQs and Protocols The graph illustrates a sample application: homogenizing up to 24 samples of chicken muscle in just 3 minutes.    Each tube had 0.2g chicken muscle in polysome lysis buffer (0.5% NP-40, 100mM KCl, 10mM HEPES, 5mM MgCl2) with 0.2 g of 0.5 mm diameter glass beads.  The samples were centrifuged to collect the protein.   Note that more protein was recovered using 0.5mL buffer (in 2.0mL conical bottom tubes) than with 1.0mL buffer (in 1.5mL tubes).  Protein levels determined by Bradford assay. Bullet Blender™ -- the cover open showing three sample tubes in place.